VITAMIN D, 25-HYDROXY

Synonyms: 25-OH-DN/A
Computer Code: VITD
Specimen Collection: 3 mL blood (light green top (Li Heparin) preferred. Red/gold acceptable).
Minimum Volume: 1.0 mL
Handling Instructions for Offsite Areas: Allow to clot, centrifuge for 20 minutes, remove serum, freeze. Min 0.2 mL serum.
Reference Values: <20 ng/ml = Deficiency 20 to <30 ng/ml = Mild Deficiency 30 - 100 ng/ml = Optimal >100 ng/ml = Possible Toxicity Note: Season, race and dietary intake are all known to affect the normal levels of 25-OH-D. 25-OH-D values correlate well with U.V. radiation exposure. Highest levels of 25-OH-D are found during the summer months and lowest levels during the winter. Race has also been shown to significantly influence the normal levels of 25-OH-D. It has been reported that the mean plasma level of 25-OH-D in whites is greater than in blacks.
Lab Code: CC
Requisition: SPEC C
Test Frequency: Daily
Routine TAT: 4 hrs
Stat TAT: 60 min
CPT Code(s): 82306
LCD or NCD: LCD LCD LCD
Methodology Used:
See Addendum XVII